Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Primer sequences used for reverse transcription polymerase chain reaction analysis of NELIN.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Primer sequences used for reverse transcription polymerase chain reaction analysis of SM22α.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Reverse transcription polymerase chain reaction analysis revealed weak mRNA expression of NELIN in the vascular smooth muscle cells (VSMCs) of the experimental group, but strong expression in the control group. (A) Expected length of the PCR products was 611 bp (NELIN) and 312 bp (β-actin). Lanes: N, control group; X, DNA marker DL 2000; and P, experimental group. (B) Downregulation of NELIN mRNA expression in the VSMCs from the varicose vein tissue. Data are presented as the mean ± standard deviation.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Reverse transcription polymerase chain reaction analysis revealed weak mRNA expression of SM22α in the vascular smooth muscle cells (VSMCs) of the experimental group, but strong expression in the control group. (A) Expected length of the PCR products was 232 bp (SM22α) and 472 bp (GADPH). Lanes: N, control group; X, DNA marker DL 2000; and P, experimental group. (B) Downregulation of SM22α mRNA expression in the VSMCs from the varicose vein tissue. Data are presented as the mean ± standard deviation.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Standard Deviation

Journal: bioRxiv

Article Title: Pitfalls in SARS-CoV-2 PCR diagnostics

doi: 10.1101/2020.06.03.132357

Figure Lengend Snippet: Real-time RT-PCR results generated by using two different batches of the identical in-house primers and probe in combination with two distinct PCR kits. RIC - RNA isolation control, NTC - no template control, PC - positive control, AgPath - AgPath-ID™ One-Step RT-PCR kit (Thermo Fisher Scientific, Germany), SSIII - SuperScript III One Step RT-PCR kit (Thermo Fisher Scientific, Germany)

Article Snippet: To exclude the PCR chemistry or the internal control oligonucleotides as potential sources of the false-positive results, samples from the first German proficiency test on COVID-19 diagnostics (INSTAND e. V. and GBD Gesellschaft für Biotechnologische Diagnostik mbH) as well as seven negative RNA isolation controls (RIC = PBS) were tested using the incriminated primers/probes in combination with two different batches of both RT-PCR kits.

Techniques: Quantitative RT-PCR, Generated, Isolation, Positive Control, Reverse Transcription Polymerase Chain Reaction

Journal: bioRxiv

Article Title: Pitfalls in SARS-CoV-2 PCR diagnostics

doi: 10.1101/2020.06.03.132357

Figure Lengend Snippet: Real-time RT-PCR results of samples that were tested either individually (black dots) or in pools consisting of one SARS-CoV-2 positive and four negative samples (blue dots). RIC - RNA isolation control, NTC - no template control, PC - positive control, AgPath - AgPath-ID™ One-Step RT-PCR kit (Thermo Fisher Scientific, Germany)

Article Snippet: To exclude the PCR chemistry or the internal control oligonucleotides as potential sources of the false-positive results, samples from the first German proficiency test on COVID-19 diagnostics (INSTAND e. V. and GBD Gesellschaft für Biotechnologische Diagnostik mbH) as well as seven negative RNA isolation controls (RIC = PBS) were tested using the incriminated primers/probes in combination with two different batches of both RT-PCR kits.

Techniques: Quantitative RT-PCR, Isolation, Positive Control, Reverse Transcription Polymerase Chain Reaction